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Calmodulin

货号 货期 规格 / 价格 询价

Calmodulin

品牌

Swant

货号

rCM

产品分类

Recombinant Protein

研究领域

Others

产品概述

Reconstitute with 10 ul bidistilled water. Freeze in small aliquots (e.g. 1 ug) and keep at -80°C. Avoid repeated freezing and thawing.

?#38382;?/p>

This recombinant protein has been purified by HPLC. This protein can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection. For adsorption we suggest the following procedure: - Dilute 1 μl of the antibody 465 (Swant) against calmodulin in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000). - Add 1 μg of the recombinant protein to 1 ml of the diluted antibody solution and mix well. - Incubate for at least 6 hours in the cold. - Apply to tissue-sections and incubate for 3 days. - Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB). As a result, the immunostaining should be strongly reduced or even completely prevented.

浓度

recombinant calmodulin produced in Escherichia Coli.

纯度

VU1

试剂准备

lyophilized (10 ug in 10 ul of: 10 mM Tris-HCl, 1 mM EGTA).

产品描述

Purification: HPLC Lot No: VU-1

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