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Calbindin D-9k

货号 货期 规格 / 价格 询价

Calbindin D-9k

品牌

Swant

货号

rCB9

产品分类

Recombinant Protein

研究领域

Calbindin

产品概述

Freeze in small aliquots (e.g. 1 or 10 ug) and keep at -80°C. Avoid repeated freezing and thawing.

浓度

Rat recombinant calbindin D-9k (recombinant) produced in Escherichia Coli.

纯度

Recombinant rat

产品描述

Purification: Ni-chelate chrom. Lot No: 5 Reconstitution: 10 ug protein reconstituted with 25 ul PBS (0.4 ug/ul) 60 ug protein reconstituted with 150 ul PBS Purification: E. coli containing a His6-fusion protein were disrupted in a buffer containing 6M GuHCl/0.1 M NaH2PO4. After a low speed centrifugation to remove cell debris, the supernatant was applied to the charged NTA resin column, equilibrated in disruption buffer, before the column being washed with the same buffer. Afterwards, the column was washed and the HIS6- fusion proteins eluted by decreasing the pH of the eluent (8 M Urea /0.1 M Na2HPO4) in a stepwise manner (pH 6.3 / 5.9 / 4 / 2). The protein was dialysed against phosphate buffered saline, 0.1 M, pH 7.3 (PBS).

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