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Rabbit anti-ALG2 8522

Mb-2-200 μl.
货号 货期 规格 / 价格 询价

Rabbit anti-ALG2 8522

品牌

Swant

货号

Mb-2-200 μl.

产品分类

Antibodies against Ca2+?-bindin

研究领域

Others

产品概述

After reconstitution freeze in small aliquots (e.g. 1 μl). For long storage, keep at - 80°C (or at least - 20°C). For continuous use keep at 4°C (with 0.01% Naazide). Avoid repeated freezing and thawing.

?#38382;?/p>

In many apoptotic pathways Ca2+ seems to play an important role. The Ca2+ binding protein ALG-2 is an apoptotic protein discovered in a death trap assay and described as a proapoptotic protein by the group of D'Adamio (1). The protein of 191 amino acids contains two canonical Ca2+ binding EF-hand domains, binding Ca2+ with high affinity and one non canonical EF-hand, binding Ca2+ with low affinity. Cells lacking ALG-2 still contain active caspases but are protected against apoptosis induced by Fas, glucocorticoids and staurosporin. The protein is expressed ubiquitously and was conserved during evolution from C.elegans to man (2). Expression of ALG-2 is increased in various cancers (3). This antiserum was made against the full-length recombinant ALG2-protein.

浓度

ALG-2 (8522) Apoptosis linked gene

纯度

Rabbit

试剂准备

Lyophilized antiserum (no preservatives).

复溶

1. Vito, P., Lacana, E. & D'Adamio, L. Science 271, 521-525 (1996). 2. Tarabykina, S., L?ss? M?ller, A., Durussel, I., Cox, J. and Berchtold, M.W., J. Biol. Chem. 275, 10514-10518(2000). 3. La Cour JM, Moellerup J., Winding P., Tarabykina S., Sehested M. and Berchtold MW, Am. J. Pathol., 163, 81-89 (2003).

应用

Immunohistochemistry: 1:2'000 - 1:5'000 with the avidin-biotin method. On paraformaldehyde (4%) or formalin-fixed tissue. Cryostate sections. Immunoblots: 1:1’000 - 1:2'000. We recommend that the optimal dilutions be determined by titration experiments.

产品描述

Lot No: 18299 Reconstitution: with 200 μl bidistilled water. Product: Antiserum

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